DNA

Part:BBa_K1588009:Design

Designed by: Juan David Sifontes   Group: iGEM15_UNAH   (2015-09-18)


Protein with epitopes from dengue virus serotype 4 to generate immune response


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1279
    Illegal PstI site found at 1432
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1279
    Illegal PstI site found at 1432
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1279
    Illegal PstI site found at 1432
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1279
    Illegal PstI site found at 1432
    Illegal NgoMIV site found at 859
    Illegal NgoMIV site found at 1690
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 938


Design Notes

1. It is coded by just one gene 2. Is not too big 3. It is exported by bacterial cells 4. Has big hydrophylic loops to insert epitopes


Source

Vibrio cholerae VC_0620

References